Cell adhesive PET membranes by surface grafting of RGD peptidomimetics.

Summary

A non-peptide mimic of the Arg-Gly Asp (RGD) active sequence of adhesive proteins (such as vitronectin) has been equipped with two different spacer-arms for surface anchorage. The covalent grafting on poly(ethylene terephthalate) (PET) membrane was realized via the activation of the hydroxyl polymer chain-ends by tosylation followed by nucleophilic substitution. The surface density of peptidomimetics was determined by X-ray photoelectron spectroscopy (XPS), on the basis of F/C atomic ratios since a fluorine tag was incorporated into the RGD-like compounds. The biological activity of soluble peptidomimetics was evaluated versus isolated human integrin alpha(v)beta(3) (vitronectin receptor), and versus CaCo2 cells. Inhibition of cellular adhesion was observed after pre-incubation of CaCo2 cells with soluble peptidomimetics. On the other hand a significant promotion of cellular adhesion resulted from the surface grafting of peptidomimetics on the PET culture substrate. The best performance was obtained with the RGD-like integrin ligand bearing a triethylene glycol spacer-arm.

Informations complémentaires

:	Accès restreint
Publication Date :	2005
:	UCL
Document type :	Article de périodique (Journal article) - (Journal Article - Research Support, Non-U.S. Gov’t)
Source :	“Biomaterials” - Vol. 26, no. 22, p. 4576-87 (2005)
Peer-reviewed :
issn :	0142-9612
Publication status :	Publié
MESH :	Cell Adhesion ; Cell Line ; Humans ; Magnetic Resonance Spectroscopy ; Membranes, Artificial ; Molecular Mimicry ; Oligopeptides - chemistry ; Surface Properties

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